Biological molecules make up all living creatures on earth and contain four major elements -- hydrogen, carbon, nitrogen and oxygen. But until now scientists were only able to use nuclear magnetic resonance (NMR) to study three out of the four elements in the molecule puzzle because oxygen wavelengths were difficult to detect.
"Oxygen signals were so weak, so to speak, that no one could make use of them," says chemistry professor Gang Wu. "Now there is a way of detecting them even in complex biomolecular systems."
Dr. Wu and his colleagues used one of the strongest NMR spectrometers in the world, located at the National Ultrahigh-Field NMR Facility for Solids in Ottawa, to create a magnetic field in which oxygen's wavelength could be detected. They also enriched the oxygen in the molecule using isotope enrichment, and implemented new NMR techniques to boost the sensitivity for detecting weak signals.
The result is an amplified oxygen wavelength that can be studied. Scientists can now examine all four major elements and learn more about the chemical structure and interaction of large molecules.
Dr. Wu's colleagues include lead author and Queen's post-doctoral fellow Jianfeng Zhu, Eric Ye (University of Ottawa) and Victor Terskikh (NRC Steacie Institute for Molecular Sciences).
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